Hexokinase (yeast)
MANUFACTURER: MEGAZYME
PRODUCT CODE; E-HEX10
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Description
Hexokinase (yeast)
Product Highlights for Hexokinase (yeast)
| Content: | 10,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.1.1 |
| CAS Number: | 9001-51-8 |
| Synonyms: | hexokinase; ATP:D-hexose 6-phosphotransferase |
| Source: | Yeast |
| Molecular Weight: | 53,738 |
| Concentration: | Supplied at ~ 1,280 U/mL |
| Expression: | From Yeast |
| Specificity: | Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. |
| Specific Activity: | ~ 100 U/mg (25oC, pH 7.4 on glucose) |
| Unit Definition: | One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
