α-Amylase (Thermostable) (Bacillus sp.)

α-Amylase (Thermostable) (Bacillus sp.)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-BSTAA

SKU: EA-261-148

Description

α-Amylase (Thermostable) (Bacillus sp.)

α-Amylase (Thermostable) (Bacillus sp.)

Content: 10 mL; 3,000 U/mL
Shipping Temperature: Ambient
Storage Temperature: 4oC
Formulation: In 50% (v/v) glycerol plus 0.02% sodium azide
Physical Form: Solution
Stability: > 1 year under recommended storage conditions

High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus licheniformis,).

Enzyme Activity: α-Amylase
EC Number: 3.2.1.1
CAZy Family: GH13
CAS Number: 9000-90-2,
9000-85-5
Synonyms: alpha-amylase; 4-alpha-D-glucan glucanohydrolase
Source: Bacillus sp.
Molecular Weight: 58,000
Concentration: Supplied at ~ 3,000 U/mL
Expression: Purified from Bacillus sp.
Specificity: Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch).
Specific Activity: ~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent)
Unit Definition: One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC.
Temperature Optima: 100oC
pH Optima: 7
Application examples: For use in carbohydrate research.
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