β-Amylase (Barley)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-BARBL-50KU

SKU: EA-261-150

Description

β-Amylase (Barley)

β-Amylase (Barley)

Content: 20,000 Units or 50,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions

Pure, crystalline suspenson. Suitable for starch structural work.

Powder enzyme form (2000°L) is for use in AACC and ASBC α-amylase assay procedures

Enzyme Activity: β-Amylase
EC Number: 3.2.1.2
CAZy Family: GH14
CAS Number: 9000-91-3
Synonyms: beta-amylase; 4-alpha-D-glucan maltohydrolase
Source: Hordeum vulgare
Molecular Weight: 58,300
Concentration: E-BARBL-20KU: Supplied at ~ 10,000 U/mL
E-BARBL-50KU: Supplied at ~ 10,000 U/mL.
Expression: From barley flour
Specificity: Hydrolysis of α-1,4-D-glucosidic linkages in polysaccharides releasing maltose units from the non-reducing end.
Specific Activity: ~ 400 U/mg (40oC, pH 6.0 on soluble starch)
Unit Definition: One Unit of β-amylase activity is defined as the amount of enzyme required to release one µmole of maltose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium phosphate buffer (200 mM), pH 6.0 at 40oC.
Temperature Optima: 60oC
pH Optima: 6
Application examples: For use in AACC and ASBC α-amylase assay procedures.
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