α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-FUCHS

SKU: EA-261-165

Description

α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

Content: 10 Units at 25oC (~ 34 Units at 37oC)
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions

High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: α-Fucosidase
EC Number: 3.2.1.51
CAZy Family: GH29
CAS Number: 9037-65-4
Synonyms: alpha-L-fucosidase; alpha-L-fucoside fucohydrolase
Source: Homo sapiens
Molecular Weight: 52,000
Concentration: Supplied at ~ 50 U/mL
Expression: Recombinant from Homo sapiens
Specificity: Broad specificity; hydrolysis of terminal non-reducing α-(1-2,3,4,6)-linked L-fucose residues from glycoproteins and oligosaccharides
Specific Activity: ~ 3 U/mg protein (pH 4.0, 25°C on pNP-α-L-fucopyranoside);
~ 14 U/mg protein (pH 4.0, 37°C on pNP-α-L-fucopyranoside);
~ 41 U/mg protein (pH 4.0, 50°C on pNP-α-L-fucopyranoside)
Unit Definition: One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-fucopyranoside (1 mM) in sodium acetate buffer (100 mM) at pH 4.0 at the temperatures indicated.
Temperature Optima: 50oC
pH Optima: 4
Application examples: For use in glycobiology research.