β-Amylase (Barley)
β-Amylase (Barley)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-BARBL-50KU
- Description
- Size Guide
- SPECIFICATION
- DATA SHEET
Description
β-Amylase (Barley)
| Content: | 20,000 Units or 50,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
Pure, crystalline suspenson. Suitable for starch structural work.
Powder enzyme form (2000°L) is for use in AACC and ASBC α-amylase assay procedures
| Enzyme Activity: | β-Amylase |
| EC Number: | 3.2.1.2 |
| CAZy Family: | GH14 |
| CAS Number: | 9000-91-3 |
| Synonyms: | beta-amylase; 4-alpha-D-glucan maltohydrolase |
| Source: | Hordeum vulgare |
| Molecular Weight: | 58,300 |
| Concentration: | E-BARBL-20KU: Supplied at ~ 10,000 U/mL E-BARBL-50KU: Supplied at ~ 10,000 U/mL. |
| Expression: | From barley flour |
| Specificity: | Hydrolysis of α-1,4-D-glucosidic linkages in polysaccharides releasing maltose units from the non-reducing end. |
| Specific Activity: | ~ 400 U/mg (40oC, pH 6.0 on soluble starch) |
| Unit Definition: | One Unit of β-amylase activity is defined as the amount of enzyme required to release one µmole of maltose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium phosphate buffer (200 mM), pH 6.0 at 40oC. |
| Temperature Optima: | 60oC |
| pH Optima: | 6 |
| Application examples: | For use in AACC and ASBC α-amylase assay procedures. |
