3-Hydroxybutyrate dehydrogenase (prokaryote)
MANUFACTURER: MEGAZYME
PRODUCT CODE; E-HBDH
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Description
3-Hydroxybutyrate dehydrogenase (prokaryote)
Product Highlights for 3-Hydroxybutyrate dehydrogenase (prokaryote)
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. |
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.30 |
| CAS Number: | 9028-38-0 |
| Synonyms: | 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase |
| Source: | Prokaryote |
| Molecular Weight: | 29,177 |
| Concentration: | Supplied at ~ 100 U/mL |
| Expression: | Recombinant from a Prokaryotic source |
| Specificity: | Catalyses the reaction: D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+ |
| Specific Activity: | ~ 77.4 U/mg (25oC, pH 8.0 on D-β-hydroxybutyric acid) |
| Unit Definition: | One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8.0 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8 |
| Application examples: | Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries. |
