Amyloglucosidase (Aspergillus niger)
$786.31
Amyloglucosidase (Aspergillus niger)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-AMGDF-A-100ML
- Description
- Size Guide
- Specification
- DATA SHEET
Description
Amyloglucosidase (Aspergillus niger)
| Content: | 10 mL or 40 mL or 100 mL or 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase and Isoamylase are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Enzyme Activity: | Amyloglucosidase |
| EC Number: | 3.2.1.3 |
| CAZy Family: | GH15 |
| CAS Number: | 9032-08-0 |
| Synonyms: | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase |
| Source: | Aspergillus niger |
| Molecular Weight: | 143,500 |
| Expression: | From Aspergillus niger |
| Specificity: | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. |
| Specific Activity: | ~ 3,260 U/mL (40oC, pH 4.5 on soluble starch) ~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside) |
| Unit Definition: | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC. |
| Temperature Optima: | 70oC |
| pH Optima: | 4 |
| Application examples: | Applications for the measurement of starch and dietary fiber in the food and feeds industry. |
