Amyloglucosidase (Aspergillus niger)

Amyloglucosidase (Aspergillus niger)

Amyloglucosidase (Aspergillus niger)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-AMGDF-40ML

SKU: EA-261-153

Description

Amyloglucosidase (Aspergillus niger)

Amyloglucosidase (Aspergillus niger)

Content: 10 mL or 40 mL or 100 mL or 100 mL (ANKOM)
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 50% (v/v) glycerol
Physical Form: Solution
Stability: > 1 year under recommended storage conditions

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase and Isoamylase are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.

Enzyme Activity: Amyloglucosidase
EC Number: 3.2.1.3
CAZy Family: GH15
CAS Number: 9032-08-0
Synonyms: glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
Source: Aspergillus niger
Molecular Weight: 143,500
Expression: From Aspergillus niger
Specificity: Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.
Specific Activity: ~ 3,260 U/mL (40oC, pH 4.5 on soluble starch)
~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside)
Unit Definition: One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC.
Temperature Optima: 70oC
pH Optima: 4
Application examples: Applications for the measurement of starch and dietary fiber in the food and feeds industry.
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