Casman Medium Base – 500g

$144.85

Manufacturer Name: NEOGEN

Manufacturer No: NCM00141A

SKU: EA-1613-606

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Description

Casman Medium Base – 500g

Name: Casman Medium Base
SKU: EA-1613-606
Price: 144.85 USD
Availability: InStock

Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.

In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.

Casman Medium Base – 500g

Specifications

Brand

Neogen® Culture Media

Analyte

Haemophilus influenzae, Neisseria gonorrhoeae

Platform

Dehydrated Culture Media

Mass

10 kg

Formula

Formula	Liter
Enzymatic Digest of Casein	5.0 g/L
Enzymatic Digest of Animal Tissue	5.0 g/L
Yeast Extract	10.0 g/L
Beef Extract	3.0 g/L
Niacinamide	0.05 g/L
p-Aminobenzoic Acid	0.05 g/L
Dextrose	0.5 g/L
Corn Starch	1.0 g/L
Sodium Chloride	5.0 g/L
Agar	13.5 g/L

Grams per Liter

43.00

Liters per 500 g

12.00

Plates per 500 g

630.00

Preparation

Suspend 43 g of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
Cool to 45-50°C and aseptically add 5% sterile blood and 0.15% sterile water-lysed blood solution (one part blood to three parts water).

Previous Item Number

Product was previously known as Acumedia Product: 7123 Casman Medium Base

Package Weight

26.60 lb