Cellobiohydrolase I (Trichoderma longibrachiatum)
$365.63
Cellobiohydrolase I (Trichoderma longibrachiatum)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-CBHI
- Description
- Size Guide
- SPECIFICATION
- DATA SHEET
Description
Cellobiohydrolase I
(Trichoderma longibrachiatum)
| Content: | 5 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
High purity Cellobiohydrolase I (Trichoderma longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Enzyme Activity: | Cellobiohydrolase |
| EC Number: | 3.2.1.176 |
| CAZy Family: | GH7 |
| CAS Number: | 37329-65-0 |
| Synonyms: | cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end) |
| Source: | Trichoderma longibrachiatum |
| Molecular Weight: | 65,000 |
| Concentration: | Supplied at ~ 0.1 U/mg |
| Expression: | Purified from Trichoderma longibrachiatum |
| Specificity: | Hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Active on pNP β-lactoside. |
| Specific Activity: | ~ 0.1 U/mg (40oC, pH 4.5 on p-nitrophenyl-β-lactoside). |
| Unit Definition: | One Unit of cellobiohydrolase I activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-lactoside (2.5 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 and 40oC. |
| Temperature Optima: | 70oC |
| pH Optima: | 4.5 |
| Application examples: | Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries. |
