Doxycycline (DOXY) is a high-efficiency broad-spectrum antibiotic with strong inhibitory effect on a variety of pathogenic bacteria, and is often used in the treatment of various infectious diseases in animals.

The Doxycycline ELISA Test Kit produced by Meizheng is fast, easy-to-use, accurate and has high sensitivity, with 45 minutes operation time, which can minimize the operating error and working intensity.

[Principles]

Doxycycline ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Doxycycline antigen is precoated on the wells. The precoated antigen compete the Doxycycline antibody (Antibody Solution) with Doxycycline in the sample, Doxycycline  antibody binds to the Doxycycline Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Doxycycline contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Doxycycline in the sample can be calculated.

DATA SHEET

[Cross Reactivity]

Doxycycline:100%

[Materials Required But Not Provided]

1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Centrifuge tube: 5mL/7mL, 50mL

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized or distilled water

—- Methanol (AR)

—- Concentrated Hydrochloric Acid (HCl) (AR)

—- 18M Concentrated Sulfuric Acid (H2SO4)

[Provided Materials and Reagent]

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagentor consult the sales manager.

[Cross Reactivity]

Doxycycline:100%

[Materials Required But Not Provided]

1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Centrifuge tube: 5mL/7mL, 50mL

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized or distilled water

—- Methanol (AR)

—- Concentrated Hydrochloric Acid (HCl) (AR)

—- 18M Concentrated Sulfuric Acid (H2SO4)

[Provided Materials and Reagent]

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.