【SPECIFICITY】
Flumequine………………………………..………………………100%
Ciprofloxacin…..……………………………..…………………>100%
Enrofloxacin…………………………………..…………………>100%
【KIT CONTENTS, STORAGE, & SHELF LIFE】
Flumequine ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.
| Kit Contents | Quantity | Storage |
| Flumequine-Coated Plate | 1 x 96-well plate | 2–8°C |
| Flumequine Standards | ||
| Negative control (white cap tube) | 1.8 mL | 2–8°C |
| 1 ng/mL (yellow cap tube) | 1.8 mL | 2–8°C |
| 5 ng/mL (orange cap tube) | 1.8 mL | 2–8°C |
| 10 ng/mL (pink cap tube) | 1.8 mL | 2–8°C |
| 25 ng/mL (purple cap tube) | 1.8 mL | 2–8°C |
| 75 ng/mL (blue cap tube) | 1.8 mL | 2–8°C |
| Spiking Solution | ||
| 1,000 ng/mL (spiking, red cap tube) | 1.8 mL | 2–8°C |
| Flumequine-HRP Conjugate | 8 mL | 2–8°C |
| 20× Wash Solution | 28 mL | 2–8°C |
| TMB Substrate | 12 mL | 2–8°C |
| 10× Sample Extraction Buffer | 25 mL | 2–8°C |
Required Materials Not Provided with the Kit
- Microtiter Plate Reader with 450 nm primary filter and optional 630 nm differential filter
- Tissue Homogenizer
- Vortex Mixer
- 10, 20, 100 and 1000 µL Pipettes
- Distilled or deionized water
- Multi-Channel Pipette: 50–300 µL (Optional)
- Reagent Resevoir (Optional)
- Incubator capable of at least 37°CIncubator
- Ethyl acetate, CAS 141-78-6 (ACS-grade or higher recommended)
- Hexane, CAS 110-54-3 (ACS-grade or higher recommended)
- Methanol, CAS 67-56-1 (ACS-grade or higher recommended)
- 18M concentrated sulfuric acid (H2SO4)
【SENSITIVITY】
| Sample Type | Detection Limit (ng/g or ppb) |
| Beef | 19 |
| Chicken | 19 |
| Fish | 0.5 |
| Shrimp | 0.5 |
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.













