Galactose dehydrogenase – Galactose mutarotase

Galactose dehydrogenase – Galactose mutarotase

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-GALMUT

SKU: EA-261-137

Description

Galactose dehydrogenase – Galactose mutarotase

Galactose dehydrogenase - Galactose mutarotase

High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Can be employed for the rapid determination of D-galactose or L-arabinose

Enzyme Activity: Dehydrogenase
EC Number: Galactose dehydrogenase: 1.1.1.48
Galactose mutarotase: 5.1.3.3
CAS Number: Galactose dehydrogenase: 9028-54-0
Galactose mutarotase: 9031-76-9
Synonyms: Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase
Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase
Source: Escherichia coli
Expression: Recombinant from Escherichia coli
Specificity: Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1).

(1) α-D-Galactose ↔ β-D-Galactose.

The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2).

(2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Specific Activity: Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC
Galactose mutarotase: ~ 4 mg/mL
Unit Definition: One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25oC.
Temperature Optima: 25oC
pH Optima: 8.6
Application examples: Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).
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