GeneArt™ Genomic Cleavage Detection Kit
$2,630.60
GeneArt™ Genomic Cleavage Detection Kit
Manufacturer: Thermo Fisher Scientific
Main Ref: 400129-MAR
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Description
GeneArt™ Genomic Cleavage Detection Kit
The GeneArt™ Genomic Cleavage Detection Kit is designed to detect and quantify targeted genome modifications resulting from site-specific nucleases such as CRISPR/Cas9, TALENs, and Zinc Finger Nucleases (ZFNs). It is commonly used to assess genome editing efficiency by detecting insertions or deletions (indels) introduced at the target site after genome editing.
Features:
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Detection Method: The kit uses a mismatch cleavage assay to identify mutations induced by genome editing. It relies on a nuclease that specifically cleaves heteroduplex DNA formed between wild-type and mutated DNA strands.
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Applications: Ideal for evaluating genome editing outcomes, including gene knockout or knock-in experiments.
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Simple Workflow: Compatible with PCR-amplified genomic DNA followed by heteroduplex formation, enzymatic cleavage, and gel or capillary electrophoresis analysis.
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Fast and Sensitive: Enables detection of low-frequency mutations without the need for cloning or sequencing.
