Goat Anti-Mouse IgG(h+l)
Price range: $220.50 through $516.53
Manufacturer: Ecalbio
Manufacturer No: CE20201 CE20206 CE20243 CE20254 CE20255 CE20256 CE20257 CE20258
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Description
Goat Anti-Mouse IgG(h+l)
General description
Immunoglobulin G (IgG) is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Recognizes mouse IgG, both heavy and light chains.
Immunogen
Highly purified whole mouse IgG.
Application
Additional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.
Research Category
Secondary & Control Antibodies
Research Subcategory
Whole Immunoglobulin Secondary Antibodies
Quality
Western Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Physical form
Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
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Additional information
| Goat Anti-Mouse | IgG(H+L), IgM (u chain), IgG1 (H+L), IgG2a (H+L), IgG2b(H+L), IgG3 (H+L), IgM (H+L), IgA (H+L) |
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