Hexokinase/Glucose-6-phosphate dehydrogenase

Hexokinase/Glucose-6-phosphate dehydrogenase

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-HKGDH

SKU: EA-261-138

Description

Hexokinase/Glucose-6-phosphate dehydrogenase

Hexokinase/Glucose-6-phosphate dehydrogenase

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Kinase
EC Number: Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49
CAS Number: Hexokinase: 9001-51-8
G6P-DH: 9001-40-5
Synonyms: Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Source: Yeast
Concentration: Hexokinase: ~ 194 U/mL
G6P-DH: ~ 665 U/mL
Expression: Hexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Specificity: Hexokinase: Catalyses the reaction:
ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Unit Definition: Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25oC.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25oC.
Temperature Optima: 40oC
pH Optima: 7.4
Application examples: Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
You've just added this product to the cart: