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BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L)
Monoclonal Mouse Anti-Human Ig Kappa

Histamine Rapid Test Kit

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$333.50

Manufacturer: Ecalbio

 

 

SKU: EA-137-357
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Description

Histamine Rapid Test Kit    

 

Principle

Histaminerapid test kit is based on lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Histaminein the sample, a visible T band will appear; T band is invisible if it has more Histaminein the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Histaminein the fruit, vegetables, grains,milk sample.

 

Technical Specification                                                

Detect limit： 1000ppb            Reaction temperature: 25℃

Detect time:   30min.

 

Materials provided                                                 

Rapid Test Card 20PCS
PBS solution     50mL

 Materials required not provided                                        

Deionized water, Ethyl acetate

Centrifuge tube:  5mL, 10mL

Micropipettors:  20~200 µL, 100~ 1000 µL

Vortex, centrifuge, balance (0.01 g), homogenizer, shaker

Sample Preparation                                              

  1. Take 5±05g of the homogenized sample, add 5mL deionized water, then add

5mL ethyl acetate , shake for 5min properly.

  1. Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 10 min.
  2. Transfer 3mL supernatant, evaporate to dryness by nitrogen at 50~60 ℃.
  3. Dissolve the dry residue in 200uL PBS solution , mix for 30s.
  4. Take 80µL for analysis.

Sample Determination                                                                                     

  1. Take out the cassette from the pack and place it horizontally.
  2. Return to room temperature (20-25℃), determine the cassette immediately.
  3. Add 2 drops(Approx 80µL) sample extraction, react for 5-10 min and read result.

Result Judgement

  1. Positive: The presence of “C” band, no matter “T”

It means that Histaminein the sample extraction is higher than 1000ppb.

  1. Negative: Both clear“C” band and “T” band appears.

It means that Histaminein the sample extractionis lower than 1000ppb .

  1. Invalid: No colored band appears in C zone, no matter whether T band

appears.

Note                                                             

  1. For best results, please strictly adhere to these instructions.
  2. All reagents must be at room temperature before running the assay.
  3. Do not remove test cassette from its pouch until immediately before use.
  4. Do not reuse the test kit.
  5. Do not use the test beyond its expiration date marked on the foil pouch.
  6. The components in this kit have been quality control tested as standard batch unit.
  7. Do not mix components from different lot numbers.

 

 

Storage and expiry date                                             

  1. Store at 4-30 °C for 12 months in the dark. Do not freeze.
  2. Expiry date is labelled on the package.

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