1. Overview:

DON belongs to the single-endomycin family of molds and is produced by certain Fusarium fungi. 3-ADON and 15-ADON are derivatives of DON. These toxins are mostly distributed in grain seeds such as wheat, barley, and corn. Due to their high cytotoxicity and immunosuppressive properties, they pose a health threat to humans and animals.

Product Properties: DON 2000ng, 3-ADON 1000ng, 15-ADON 2000ng;

Specification: 3mL,50T

Samples: Grain, oil, feed, alcohol, condiments, etc.

2. Principle:

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the aflatoxin in the sample is extracted, filtered, and diluted, and then passed slowly through the aflatoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Deoxynivalenol is then eluted with methanol and injected into an analytical instrument for detection.

3. Components of the kit:

Each kit contains Deoxynivalenol immunoaffinity columns of various specifications and 1 instruction manual.

Necessary items not provided in the box:

Equipment

HPLC/LC-MS/MS

Nitrogen gas evaporator apparatus

Nitrogen gas tank and pressure regulator

Air-pressure controller bracket

Air pump

Balance with 0.01g readability

High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker

Grinder

Sieving screen:1-mm

Graduated cylinder: 100 mL/10 mL

Funnel: 50 mL

Syringe: 10 mL/20 mL

Pipette: 1 mL and pipette tips

Homogenization flask (or 250-mL conical flask with pestle)

Vials and tubes

Rapid qualitative filter paper

Microfiber filter paper (e.g., Whatman 934-AH)

Column holder and syringe connector plug (for use with immunoaffinity columns)

4. Reagents

Methanol (CH3OH): Analytical grade for extraction/chromatography grade for elution

Acetonitrile (CH3CN): Analytical Grade

Sodium chloride (NaCl): Analytical Grade

Potassium dihydrogen phosphate (KH2PO4): Analytical Grade

Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade

Polyethylene glycol (8000Da, HO(CH2CH2O)nH): Analytical Grade, to improve filtration speed, if the supernatant is obtained by centrifugation , this reagent can be omitted.

TWEEN-20 (C58H114O26): Analytical Grade

Hydrochloric acid (HCl): Analytical Grade

Sodium hydroxide (NaOH): Analytical Grade

Potassium chloride (KCl): Analytical Grade

Distilled or deionized water

5. Note:

Allow the immunoaffinity column to return to room temperature (22 to 25°C) before use.

The affinity column should be stored at 2 to 8°C, do not freeze.

Dot use any expired immunoaffinity column.

The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted accordingly.

6. Column capacity

6.1 The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.

6.2 Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent effects.

6.3 WARNING: Aflatoxin is toxic and carcinogenic; protective equipment such as gloves and masks should always be used during handling.