1. Overview:

The basis of the measurement is the antigen-antibody  reaction. More than 20 antibodies of seven main toxins are connected to the column concurrently. After the sample is extracted and filtered, it is slowly passed through the immunoaffinity column. The toxins bind to the corresponding antibodies in the column. The immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Elute the toxin with eluent and then inject into the analytical instrument for detection.

Order No. : HCM4210;

Product Properties: AFT B1/B2/G1/G2/M1/M2: 300, FB1/FB2/FB3: 5000, OTA: 100, ST:300, DON/15-ADON: 2000, ZEN: 1000, T-2/HT-2:1000;

Specification: 6mL,10T;

Samples: Grain,feed,etc.

2. Components of the kit:

Each kit contains 7 in 1 immunoaffinity columns and 1 instruction manual.

Necessary items not provided in the box:

Equipment

LC-MS/MS

Nitrogen gas evaporator apparatus

Nitrogen gas tank and pressure regulator

Air-pressure controller bracket

Air pump

Balance with 0.01g readability

High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker

Grinder

Sieving screen:2-mm

Graduated cylinder: 100 mL/10 mL

Funnel: 50 mL

Syringe: 10 mL/20 mL

Pipette: 1 mL and pipette tips

Homogenization flask (or 250-mL conical flask with pestle)

Sample bottles and tubes

Rapid qualitative filter paper

Microfiber filter paper (e.g. Whatman 934-AH)

Column holder and syringe connector plug (for use with immunoaffinity columns)

3. Reagents

Methanol (CH3OH): Chromatography Grade

Acetic acid (CH3COOH): Analytical Grade

Acetonitrile (CH3CN): Analytical Grade

Sodium chloride (NaCl): Analytical Grade

TWEEN-20 (C58H114O26): Analytical Grade

Sodium hydroxide (NaOH): Analytical Grade

Potassium chloride (KCl): Analytical Grade

Potassium dihydrogen phosphate (KH2PO4): Analytical Grade

Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade

Distilled or deionized water