Lichenase (endo-1,3:1,4-β-D-Glucanase) (Bacillus subtilis)
$758.00
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-LICHN
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Description
Lichenase (endo-1,3:1,4-β-D-Glucanase)
(Bacillus subtilis)
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
High purity Lichenase (endo-1,3,1,4-β-Glucanase) (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Enzyme Activity: | β-Glucanase/Lichenase |
| EC Number: | 3.2.1.73 |
| CAZy Family: | GH16 |
| CAS Number: | 37288-51-0 |
| Synonyms: | licheninase; (1→3)-(1→4)-beta-D-glucan 4-glucanohydrolase |
| Source: | Bacillus subtilis |
| Molecular Weight: | 26,750 |
| Concentration: | Supplied at ~ 1,000 U/mL |
| Expression: | Purified from Bacillus subtilis |
| Specificity: | Hydrolysis of (1,4)-β-D-glucosidic linkages in β-D-glucans containing (1,3)- and (1,4)-bonds. |
| Specific Activity: | ~ 250 U/mg (40oC, pH 6.5 on barley β-glucan) |
| Unit Definition: | One Unit of lichenase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100mM), pH 6.5 at 40oC. |
| Temperature Optima: | 60oC |
| pH Optima: | 6 |
| Application examples: | Applications in carbohydrate research and in the food and feeds, brewing and biofuels industries. |
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