Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

MANUFACTURER: MEGAZYME

PRODUCT CODE: K-MBG4

SKU: EA-261-74

Description

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

 

Content: (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or
(Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: β-Glucanase/Lichenase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or
(Lichenase) 9.1 x 10-5 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: (Malt β-glucanase) ~ 20 min Or
(Lichenase) ~ 10 min
Application examples: Crude malt extracts, industrial enzyme preparations.
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