Mannitol dehydrogenase (Pseudomona fluorescens)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-MNHPF
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Description
Mannitol dehydrogenase (Pseudomona fluorescens)
Product Highlights for Mannitol dehydrogenase (Pseudomona fluorescens)
| Content: | 500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.67 |
| CAS Number: | 9001-65-4 |
| Synonyms: | mannitol 2-dehydrogenase; D-mannitol:NAD+ 2-oxidoreductase |
| Source: | Pseudomona fluorescens |
| Molecular Weight: | 56,661 |
| Concentration: | Supplied at ~ 300 U/mL |
| Expression: | Recombinant from Pseudomona fluorescens |
| Specificity: | Catalyses the reaction: D-Mannitol + NAD+ = D-fructose + NADH + H+ |
| Specific Activity: | ~ 50 U/mg (40oC, pH 8.6 on D-mannitol) |
| Unit Definition: | One Unit of mannitol dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-mannitol (5.2 mM) in Tris-HCl buffer (64 mM), pH 8.6 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 8.6 |
| Application examples: | Low Km, suitable for D-mannitol determination. Potential uses in the biotechnology industry. |
