Monoclonal Mouse Anti-Human IgG（FC）
$220.50
Manufacturer: ECALBIO
Manufacturer Ref No: CE10202
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Description
Monoclonal Mouse Anti-Human IgG（FC）
General description
Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family and have two heavy (H) and two light (L) chain, held together by disulphide linkages. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.
Specificity
The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.
Application
Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- imprint immunofixation (IIF)
- immunofluorometric assay (IFMA)
- hemagglutination (HA)
- hemagglutination inhibition (HAI)
- particle counting immunoassay(PACIA)
Packaging
0.2, 0.5 mL in glass bottle
Biochem/physiol Actions
Although the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess a “Fc receptor”. These functions indicate the importance of immunoreagents specific for the Fc fragment of human IgG.
Biological source : mouse
Quality Level : 200
conjugate : unconjugated
antibody form: ascites fluid
antibody product type : secondary antibodies
clone : HP-6017, monoclonal
contains : 15 mM sodium azide
Specific Reactivity: sheep, horse (IgG), rabbit, goat, human
technique(s) : indirect ELISA: 1:3,000
isotype ; IgG2a
shipped in : dry ice
storage temp :−20°C
