Monoclonal Mouse Anti-Human IgM(u)
$220.50
Manufacturer: ECALBIO
Manufacturer Ref No: CE10204
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Description
Monoclonal Mouse Anti-Human IgM(u)
Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgM protein. Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocytes membranes. IgM has a pentameric structure, in which monomers are linked together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells.
Specificity
Monoclonal Anti-Human IgM (μ-chain) specifically recognizes μ chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, kappa, lambda and gamma chains.
Immunogen
purified human IgM protein
Application
Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse may suitable for use in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
IgM is suggested to act as the first line of defense during microbial infections. Secreted IgM (sIgM) is involved in B cell maturation and complement activation. Detection of IgM antibodies in a patient′s serum may indicates a recent infection and IgM in a neonate′s serum may indicate on intrauterine infection (e.g. congenital rubella syndrome). In addition, the development of anti-donor IgM after combined liver-kidney transplantation has been shown to provide a graft- protecting effect. Anti-Human IgM (μ-specific) antibodies can be used in detection of the abnormal concentration of IgM in patient sera and IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMD) and the hyperimmunoglobulin M (hyper-IgM or HIGM) syndromes.
Physical form
Supplied as a lyophilized powder
Storage and Stability
Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.
Biological source : mouse
Quality Level: 200
conjugate: peroxidase conjugate
antibody form : purified immunoglobulin
antibody product type : primary antibodies
clone : MB-11, monoclonal
form : lyophilized powder
species reactivity : human
concentration : ~2.0 mg/mL
technique(s) : ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating
isotype : IgG2b
shipped in : dry ice
storage temp : −20°C
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