Nutrient medium DRCM-Broth, dehydrated
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Nutrient Medium
Type: DRCM-Broth, dehydrated
Manufacturer: Dr. Möller & Schmelz GmbH (M&S)
Item Number: 5160
Packaging: 25x50ml
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Description
NUTRIENT MEDUIM-
DRCM-Broth, dehydrated
DESCRIPTION AND APPLICATION RANGE
DRCM broth is used for detection and isolation of Clostridium perfringens in water and other samples. The strictly anaerobic spore-forming Clostridia sp. are able to reduce sulfite to sulfide that forms iron sulfide, which makes the medium turn black. As other microorganisms can also produce sulfide, a pasteurization step is necessary to eliminate the vegetative cells and to prevent false positive results. For details please see the instruction for use. Resazurin is a redox indicator and used to monitor the anaerobic conditions in the medium. The medium is manufactured and quality tested in compliance withDIN EN ISO 11133:2014 standard
Profile: Plastic bottles and glass tubes
Storage: Dark and dry at 4-12°C
Colour/Appearance: Granulate waxed with paraffin
Shelf life: 6 months
INTRODUCTION
M&S sterile ready-to-use media are filled in autoclavable and break-proof polycarbonate bottles (50 ml and 250 ml) or glass tubes (20 ml). The composition of the various types of media, comply, like the NPS with the formulations specified in the pertinent standards and regulations.
Whereas the liquid media can be used immediately, the agar media first have to be remelted in a water bath at 90 – 100 °C. Once they have cooled down to around 50 °C you can pour agar plates in different sizes and thicknesses to meet your individual demands.
The shelf life of the ready-to-use media in unopened containers is 6 months.
Generally the M&S ready-to-use media are offered in three different pack sizes.
Standard pack sizes:
- 4 x 250 ml polycarbonate bottles
- 25 x 50 ml polycarbonate bottles
- 25 x 20 ml glass tubes
QUALITY ASSURANCE
Just like the nutrient pad sets, the production processes and quality control for our ready-to-use nutrient media
are integrated in the M&S quality management system and are based on the GMP guidelines.
The broth and agar media are produced using components from certified suppliers only. The weighed portions
of the individual components are documented with their batch numbers to guarantee their traceability
and the adjustment of the pH-value is also recorded. Careful filling and steriliZation ensures that the quality
of the individual nutrient media batches remain unaffected.
During the final control of the end products, sterility, recovery rates and if necessary any colour reactions
with positive and negative microorganisms are tested. The nutrient media only leave our premises if they fulfil
all of the requirements.
The batch-related M&S quality certificate that is enclosed with every package confirms that M&S products
have been checked according to our quality standards and that they meet these.
