Nutrient Pad Sets -Lysine-NPS
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Nutrient Pad Sets
Type: Lysine-NPS
Manufacturer: Dr. Möller & Schmelz GmbH (M&S)
Item Number: 1095-H
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Description
NUTRIENT PAD SETS –
Brettanomyces-NPS
NPS for the detection of yeasts and moulds
Just like certain bacteria, yeasts and moulds play an important role in the production of food, for example beer, wine, cheese and bakery products. However, some representatives are unwanted in certain production processes since they lead to off-flavours, have a negative effect on the shelf life of the food or can even cause serious health problems for consumers. Production processes thus have to be monitored with respect to these microorganisms.
DESCRIPTION AND APPLICATION RANGE
Lysine-NPS are used for the determination and colony count of “wild yeasts” in beverages, i.e. in beer. The formulation is acc. to Morris and Eddy, modified. Wild yeasts are able to use lysine as sole source of nitrogen. Pitching yeasts used e.g. in the brewery industry do not have that ability. Hence, the detection of a wild yeasts contamination within a yeast culture sample is possible by using Lysine-NPS. Important is that after filtration of the sample and before placing the membrane onto the pad the membrane filter is washed with sterile saline or another nitrogen-free buffer, in order to eliminate all present nitrogen residues. The low pH-value inhibits the development of accompanying bacteria. The medium is manufactured and quality tested in compliance with DIN EN ISO 11133:2014 standard.
Profile: Dehydrated nutrient pad sets 50 mm in petri dishes, sterile
Storage: Dark and dry at room temperature
Colour: Beige.
Shelf life: 2 years after sterilization
Application: For the selective detection of “wild yeasts” in breweries
Recommended Membrane Filter: Green with grid, pore size 0.8µm
Recommended Incubation Condition: 2 – 5 days at 25 – 30 °C
Growth: Only wild yeasts can use lysine as a sole source of nitrogen. They generally form white to cream-coloured, but occasionally also reddish colonies. Culture yeasts display no growth.
INTRODUCTION
Nutrient pad sets (NPS) are sterile dehydrated nutrient culture media that are ready for immediate use after addition of sterile water. They are made of biologically inert cellulose cardboard that serves as a substrate for the nutrient solution. Since it does not bind the nutrients either chemically or physically, the nutrient components are completely available for the growth of microorganisms. The composition of the various nutrient solutions used for the various NPS comply with the formulations specified in the relevant standards and regulations. Nutrient pad sets have a number of advantages over conventional agar media.
- Storage at room temperature
- Shelf life up to 2 years after sterilization
- Ready for immediate use after moistening
- Easy handling
The nutrient pads are offered as a complete set in 2 different pack sizes with 50 or 100 pieces.
The packaging units contain the sterile nutrient pads in sterile Petri dishes and sterile, individually packed membrane filters, with a diameter of 50 mm. Your requirements regarding the type of membrane filter or the alternative diameter of 47 mm can be considered
The number of mesophilic, heterotrophic growing bacteria provides an indication of the general hygiene status of samples.
Different nutrient media are recommended or specified for their determination depending on various regulations, standards or internal standards.
QUALITY ASSURANCE
Both the manufacturing process as well as the quality controls for the nutrient pad sets are integrated in the M&S
quality management system according to DIN EN ISO 9001:2008 and are based on GMP guidelines.
Each batch of cellulose cardboard that is used as substrate for the NPS is tested for thickness
(according to DIN EN ISO 20534), grammage (according to DIN EN ISO 536) and water absorption capacity (in-house test method).
It is also tested for growth-inhibiting substances.
The Petri dishes in which the impregnated nutrient pads are placed are manufactured under clean room conditions
and their sterility is checked regularly.
The impregnation solutions are produced using components from certified suppliers only. The weighed portions of the individual components
of the formulations are documented with their batch numbers to guarantee their traceability. The adjustment of the pH-value is also recorded.
Processing continues as soon as the solutions have been produced, i.e. the cardboard pads are then impregnated.
The drying process that follows impregnation takes place under controlled conditions.
A service provider certified in accordance with EN ISO 13485:2003 performs the sterilization of the NPS
in a validated process.
During the final inspection of the finished products, their sterility, recovery rates and if necessary
any colour reactions with positive and negative microorganisms are tested. The NPS only leave our premises if they fulfil all of the requirements.
The batch-related M&S quality certificate that accompanies every package confirms that the M&S quality standards have been checked and met.
Vendor Information
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Chemicals, Laboratory Reagents & Standards, Chemicals, Laboratory ChemicalsBRAIN HEART INFUSION BROTH
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