m-Green Yeast & Mould-NPS
m-Green Yeast & Mould-NPS

Nutrient Pad Sets -m-Green Yeast & Mould-NPS

Nutrient Pad Sets -OGY-NPS Nutrient Pad Sets -OGY-NPS
m-Green Yeast & Mould-NPS Nutrient Pad Sets -m-Green Yeast & Mould-NPS

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Nutrient Pad Sets

Type: m-Green Yeast & Mould-NPS

Manufacturer: Dr. Möller & Schmelz GmbH (M&S)

Item Number: 1105-H

SKU: EA-5310-60
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Description

NUTRIENT PAD SETS –

m-Green Yeast & Mould-NPS

NPS for the detection of yeasts and moulds

Just like certain bacteria, yeasts and moulds play an important role in the production of food, for example beer, wine, cheese and bakery products. However, some representatives are unwanted in certain production processes since they lead to off-flavours, have a negative effect on the shelf life of the food or can even cause serious health problems for consumers. Production processes thus have to be monitored with respect to these microorganisms.

DESCRIPTION AND APPLICATION RANGE

m-Green Yeast & Mould-NPS are used for the detection and colony count of yeast and molds in beer, wine, soft drinks and other beverages. The complex nutrient composition provides optimal growth conditions for yeast and moulds. The low pH supports their development and at the same time slightly inhibits the growth of accompanying bacteria. Due to the pH-indicator bromocresolgreen the colonies appear greenish and can turn to beige, if the pH further drops down. The medium is manufactured and quality tested in compliance with DIN EN ISO 11133:2014 standard

Profile:  Dehydrated nutrient pad sets 50 mm in petri dishes, sterile

Storage: Dark and dry at room temperature

Colour: Beige.

Shelf life: 2 years after sterilization

Application:For the detection of yeasts and moulds in sugar and beverages containing sugar.

Recommended Membrane Filter: Green with grid, pore size 1.2µm

Recommended Incubation Condition: 2 – 3 days at 25 – 30 °C

Growth: The acid produced by the fermentation of sugar through yeasts and moulds turns the pH-indicator from blue-green to yellow and leads to yellowish colonies, some with a yellow halo. Non-acid-formers, on the other hand, are blue-green. The low pH-value of the medium inhibits the growth of most bacteria and supports the development of yeasts and moulds.

INTRODUCTION

Nutrient pad sets (NPS) are sterile dehydrated nutrient culture media that are ready for immediate use  after addition of sterile water. They are made of biologically inert cellulose cardboard that serves as a substrate  for the nutrient solution. Since it does not bind the nutrients either chemically or physically,  the nutrient components are completely available for the growth of microorganisms. The composition of the various nutrient solutions used for the various NPS comply with the formulations specified  in the relevant standards and regulations. Nutrient pad sets have a number of advantages over conventional agar media.

  • Storage at room temperature
  • Shelf life up to 2 years after sterilization
  • Ready for immediate use after moistening
  • Easy handling

The nutrient pads are offered as a complete set in 2 different pack sizes with 50 or 100 pieces.
The packaging units contain the sterile nutrient pads in sterile Petri dishes and sterile, individually packed membrane filters, with a diameter of 50 mm. Your requirements regarding the type of membrane filter or the alternative diameter of 47 mm can be considered

The number of mesophilic, heterotrophic growing bacteria provides an indication of the general hygiene status of samples.
Different nutrient media are recommended or specified for their determination depending on various regulations, standards or internal standards.

QUALITY ASSURANCE

Both the manufacturing process as well as the quality controls for the nutrient pad sets are integrated in the M&S
quality management system according to DIN EN ISO 9001:2008 and are based on GMP guidelines.

Each batch of cellulose cardboard that is used as substrate for the NPS is tested for thickness
(according to DIN EN ISO 20534), grammage (according to DIN EN ISO 536) and water absorption capacity (in-house test method).
It is also tested for growth-inhibiting substances.

The Petri dishes in which the impregnated nutrient pads are placed are manufactured under clean room conditions
and their sterility is checked regularly.

The impregnation solutions are produced using components from certified suppliers only. The weighed portions of the individual components
of the formulations are documented with their batch numbers to guarantee their traceability. The adjustment of the pH-value is also recorded.
Processing continues as soon as the solutions have been produced, i.e. the cardboard pads are then impregnated.

The drying process that follows impregnation takes place under controlled conditions.

A service provider certified in accordance with EN ISO 13485:2003 performs the sterilization of the NPS
in a validated process.

During the final inspection of the finished products, their sterility, recovery rates and if necessary
any colour reactions with positive and negative microorganisms are tested. The NPS only leave our premises if they fulfil all of the requirements.

The batch-related M&S quality certificate that accompanies every package confirms that the M&S quality standards have been checked and met.

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