Nutrient Pad Sets -Orange Serum-NPS
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Nutrient Pad Sets
Type: Orange Serum-NPS
Manufacturer: Dr. Möller & Schmelz GmbH (M&S)
Item Number: 1120-H
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Description
NUTRIENT PAD SETS –
Orange Serum-NPS
NPS for the detection of special microorganisms or groups of microorganisms
DESCRIPTION AND APPLICATION RANGE
Orange Serum-NPS are used for the detection and colony count of acidophilicand acidotolerant microorganisms in fruit juices, soft drinks and other beverages. The composition of this medium, i.e. the presence of orange serum, together with the low pH provides optimal growth conditions for acidophilic and acid tolerant microorganisms from beverages containing fruit juices or fruit components. The growth of accompanying microorganisms is slightly inhibited by the low pH. Incubation under anaerobic or microaerophilic conditions stimulates the growth of more demanding Lactobacilli. The medium is manufactured and quality tested in compliance with DIN ENISO 11133:2014 standard.
Profile: Dehydrated nutrient pad sets 50 mm in petri dishes, sterile
Storage: Dark and dry at room temperature
Colour: Beige
Shelf life: 2 years after sterilization
Application: For the detection of acidophilic and acidotolerant microorganisms in beverages and food.
Recommended Membrane Filter: Green with grid, pore size 0.45µm
Recommended Incubation Condition: 2 – 3 days at 30 °C
Growth: Due to the composition and the low pH-value, both yeasts and moulds as well as acidotolerant bacteria can grow. Demanding lactobacilli can also develop under anaerobic incubation condition
INTRODUCTION
Nutrient pad sets (NPS) are sterile dehydrated nutrient culture media that are ready for immediate use after addition of sterile water. They are made of biologically inert cellulose cardboard that serves as a substrate for the nutrient solution. Since it does not bind the nutrients either chemically or physically, the nutrient components are completely available for the growth of microorganisms. The composition of the various nutrient solutions used for the various NPS comply with the formulations specified in the relevant standards and regulations. Nutrient pad sets have a number of advantages over conventional agar media.
- Storage at room temperature
- Shelf life up to 2 years after sterilization
- Ready for immediate use after moistening
- Easy handling
The nutrient pads are offered as a complete set in 2 different pack sizes with 50 or 100 pieces.
The packaging units contain the sterile nutrient pads in sterile Petri dishes and sterile, individually packed membrane filters, with a diameter of 50 mm. Your requirements regarding the type of membrane filter or the alternative diameter of 47 mm can be considered
The number of mesophilic, heterotrophic growing bacteria provides an indication of the general hygiene status of samples.
Different nutrient media are recommended or specified for their determination depending on various regulations, standards or internal standards.
QUALITY ASSURANCE
Both the manufacturing process as well as the quality controls for the nutrient pad sets are integrated in the M&S
quality management system according to DIN EN ISO 9001:2008 and are based on GMP guidelines.
Each batch of cellulose cardboard that is used as substrate for the NPS is tested for thickness
(according to DIN EN ISO 20534), grammage (according to DIN EN ISO 536) and water absorption capacity (in-house test method).
It is also tested for growth-inhibiting substances.
The Petri dishes in which the impregnated nutrient pads are placed are manufactured under clean room conditions
and their sterility is checked regularly.
The impregnation solutions are produced using components from certified suppliers only. The weighed portions of the individual components
of the formulations are documented with their batch numbers to guarantee their traceability. The adjustment of the pH-value is also recorded.
Processing continues as soon as the solutions have been produced, i.e. the cardboard pads are then impregnated.
The drying process that follows impregnation takes place under controlled conditions.
A service provider certified in accordance with EN ISO 13485:2003 performs the sterilization of the NPS
in a validated process.
During the final inspection of the finished products, their sterility, recovery rates and if necessary
any colour reactions with positive and negative microorganisms are tested. The NPS only leave our premises if they fulfil all of the requirements.
The batch-related M&S quality certificate that accompanies every package confirms that the M&S quality standards have been checked and met.
