Description

NUTRIENT PAD SETS –

Wine-NPS

NPS for the detection of lactic acid bacteria

Lactic acid bacteria are a heterogeneous group of anaerobic, but usually aerotolerant bacteria, all of which are able to ferment glucose to lactic acid. Although lactic acid bacteria play an important role in the production of food, the metabolic products of some representatives are unwanted in products from the beverages industry since they lead to off-flavours and spoilage. Routine tests for lactic acid bacteria in the production processes thus play an important role.

DESCRIPTION AND APPLICATION RANGE

Wine-NPS are used for detection of wine-spoiling bacteria, e.g. Lactobacillus sp., Leuconostoc sp. and Pediococcus sp. It is an improved tomato juice medium. Actidione (Cycloheximide) isused to inhibit the growth of yeasts and molds. The medium is manufactured and quality tested in compliance with DIN EN ISO 11133:2014 standard.

Profile:  Dehydrated nutrient pad sets 50 mm in petri dishes, sterile

Storage: Dark and dry at room temperature

Colour: Beige

Shelf life: 2 years after sterilization

Application: For the selective detection of wine-spoiling bacilli, including the genera Leuconostoc, Lactobacillus and Pediococcus. Acetobacter can also be detected in anaerobic conditions.

Recommended Membrane Filter: Green with grid, pore size 0.45 µm

Recommended Incubation Condition: 2 – 5 days at 30 °C under anaerobic conditions

Growth: The growth of yeasts and moulds is suppressed by Actidione.

INTRODUCTION

Nutrient pad sets (NPS) are sterile dehydrated nutrient culture media that are ready for immediate use  after addition of sterile water. They are made of biologically inert cellulose cardboard that serves as a substrate  for the nutrient solution. Since it does not bind the nutrients either chemically or physically,  the nutrient components are completely available for the growth of microorganisms. The composition of the various nutrient solutions used for the various NPS comply with the formulations specified  in the relevant standards and regulations. Nutrient pad sets have a number of advantages over conventional agar media.

• Storage at room temperature
• Shelf life up to 2 years after sterilization
• Ready for immediate use after moistening
• Easy handling

The nutrient pads are offered as a complete set in 2 different pack sizes with 50 or 100 pieces.
The packaging units contain the sterile nutrient pads in sterile Petri dishes and sterile, individually packed membrane filters, with a diameter of 50 mm. Your requirements regarding the type of membrane filter or the alternative diameter of 47 mm can be considered.

QUALITY ASSURANCE

Both the manufacturing process as well as the quality controls for the nutrient pad sets are integrated in the M&S
quality management system according to DIN EN ISO 9001:2008 and are based on GMP guidelines.

Each batch of cellulose cardboard that is used as substrate for the NPS is tested for thickness
(according to DIN EN ISO 20534), grammage (according to DIN EN ISO 536) and water absorption capacity (in-house test method).
It is also tested for growth-inhibiting substances.

The Petri dishes in which the impregnated nutrient pads are placed are manufactured under clean room conditions
and their sterility is checked regularly.

The impregnation solutions are produced using components from certified suppliers only. The weighed portions of the individual components
of the formulations are documented with their batch numbers to guarantee their traceability. The adjustment of the pH-value is also recorded.
Processing continues as soon as the solutions have been produced, i.e. the cardboard pads are then impregnated.

The drying process that follows impregnation takes place under controlled conditions.

A service provider certified in accordance with EN ISO 13485:2003 performs the sterilization of the NPS
in a validated process.

During the final inspection of the finished products, their sterility, recovery rates and if necessary
any colour reactions with positive and negative microorganisms are tested. The NPS only leave our premises if they fulfil all of the requirements.

The batch-related M&S quality certificate that accompanies every package confirms that the M&S quality standards have been checked and met.