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Perfringens Agar Base (TSC) – 500g

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$141.24

Manufacturer Name: NEOGEN

Manufacturer No: NCM0077A

 

 

 

 

 

 

SKU: EA-1613-448
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Description

Perfringens Agar Base (TSC) – 500g

Perfringens Agar Base (TSC) – 500g

Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans.

Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.

Perfringens Agar Base (TSC) – 500g

Specifications
Brand Neogen® Culture Media
Analyte Clostridium perfringens
Platform Dehydrated Culture Media
Mass 5 kg
Formula
Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soya 5.0 g/L
Yeast Extract 5.0 g/L
Sodium Metabisulfite 1.0 g/L
Ferric Ammonium Citrate 1.0 g/L
Agar 14.0 g/L
Grams per Liter 41.00
Liters per 500 g 11.00
Plates per 500 g 590.00
Preparation
  1. Suspend 41 grams of the medium in 900 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Add 100 mL of NCM4017* Egg Yolk Emulsion, 50%, and the desired antimicrobic supplement: TSC Agar: Add 2 vials of NCM4022-0.5* TSC Perfringens Supplement, each reconstituted using 5 mL sterile deionized/RO water.

Overlay Agar

  1. Suspend 41 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Overlay agar does not get supplemented with Egg Yolk Emulsion. Overlay agar is held at 45 -50°C until used and should be poured right after inoculation.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.
Previous Item Number Product was previously known as Acumedia Product: 9188 Clostridium perfringens Agar Base; and LabM Product: LAB194 Perfringens Agar (TSC)
Package Weight 15.30 lb

https://www.neogen.com/en/categories/microbiology/perfringens-agar-base/#product-docs-tab-5

https://www.neogen.com/en/categories/microbiology/perfringens-agar-base/#product-docs-tab-8

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