Phosphoglucose isomerase (Bacillus subtilis)

Phosphoglucose isomerase (Bacillus subtilis)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-PGIBS-5KU

SKU: EA-261-139

Description

Phosphoglucose isomerase (Bacillus subtilis)

Phosphoglucose isomerase (Bacillus subtilis)

High purity recombinant Phosphoglucose isomerase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Isomerase
EC Number: 5.3.1.9
CAS Number: 9001-41-6
Synonyms: glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase
Source: Bacillus subtilis
Molecular Weight: 50,500
Concentration: E-PGIBS-5KU: Supplied at > 1,000 U/mL.
E-PGIBS-50KU: Supplied at ~ 3,550 U/mL.
Expression: Recombinant from Bacillus Subtilis
Specificity: Catalyses the reaction:
D-Glucose 6-phosphate = D-fructose 6-phosphate
Specific Activity: > 60 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate);
> 120 U/mg (40oC, pH 7.6 on fructose 6-phosphate)
Unit Definition: One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate at pH 7.6.
Temperature Optima: 40oC
pH Optima: 7.6
Application examples: Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
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