Phosphoglucose isomerase (Escherichia coli)

Phosphoglucose isomerase (Escherichia coli)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-PGIEC-50KU

SKU: EA-261-140

Description

Phosphoglucose isomerase (Escherichia coli)

Phosphoglucose isomerase (Escherichia coli)

High purity Phosphoglucose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Isomerase
EC Number: 5.3.1.9
CAS Number: 9001-41-6
Synonyms: glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase
Source: Escherichia coli
Molecular Weight: 62,600
Concentration: E-PGIEC-10KU: Supplied at ~ 1,000 U/mL.
E-PGIEC-50KU: Supplied at ~ 10,000 U/mL.
Expression: Recombinant from Escherichia coli
Specificity: Catalyses the reaction:
D-Glucose 6-phosphate = D-fructose 6-phosphate
Specific Activity: ~ 655 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate);
~ 785 U/mg of protein (40oC, pH 7.6 on fructose 6-phosphate)
Unit Definition: One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.6.
Temperature Optima: 40oC
pH Optima: 7.6
Application examples: Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries
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