Phosphoglucose isomerase (Saccharomyces cerevisiae)
Phosphoglucose isomerase (Saccharomyces cerevisiae)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-PGISC-5KU
- Description
- Size Guide
- Specification
- DATA SHEET
Description
Phosphoglucose isomerase
(Saccharomyces cerevisiae)
High purity Phosphoglucose isomerase (Saccharomyces cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Enzyme Activity: | Isomerase |
| EC Number: | 5.3.1.9 |
| CAS Number: | 9001-41-6 |
| Synonyms: | glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase |
| Source: | Saccharomyces cerevisiae |
| Molecular Weight: | 62,400 |
| Concentration: | E-PGISC-5KU: Supplied at ~ 1,000 U/mL. E-PGISC-50KU: Supplied at ~ 5,000 U/mL. |
| Expression: | Recombinant from Saccharomyces cerevisiae |
| Specificity: | Catalyses the reaction: D-Glucose 6-phosphate = D-fructose 6-phosphate |
| Specific Activity: | ~ 350 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate); ~ 570 U/mg of protein (40oC, pH 7.6 on fructose 6-phosphate) |
| Unit Definition: | One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate at pH 7.6. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
