Phosphomannose isomerase (Escherichia coli)

Phosphomannose isomerase (Escherichia coli)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-PMIEC

SKU: EA-261-142

Description

Phosphomannose isomerase (Escherichia coli)

Phosphomannose isomerase (Escherichia coli)

Content: 1,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions

 

High purity recombinant Phosphomannose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Isomerase
EC Number: 5.3.1.8
CAS Number: 9023-88-5
Synonyms: mannose-6-phosphate isomerase; D-mannose-6-phosphate aldose-ketose-isomerase
Source: Escherichia coli
Molecular Weight: 43,915
Concentration: Supplied at ~ 500 U/mL
Expression: Recombinant from Escherichia coli
Specificity: Catalyses the reaction:
D-Mannose 6-phosphate = D-fructose 6-phosphate
Specific Activity: ~ 100 U/mg (25oC, pH 7.6 on mannose 6-phosphate)
Unit Definition: One Unit of phosphomannose isomerase activity is defined as the amount of enzyme required to release one µmole of fructose 6-phosphate from mannose 6-phosphate (3.14 mM) per minute in the presence of NAD+ at pH 7.6 and 25oC.
Temperature Optima: 40oC
pH Optima: 7.6
Application examples: Applications for the measurement of D-mannose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
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