Phosphomannose isomerase (Escherichia coli)
$470.92
Phosphomannose isomerase (Escherichia coli)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-PMIEC
- Description
- Size Guide
- Specification
- DATA SHEET
Description
Phosphomannose isomerase (Escherichia coli)
Content: | 1,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 1 year under recommended storage conditions |
High purity recombinant Phosphomannose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Enzyme Activity: | Isomerase |
EC Number: | 5.3.1.8 |
CAS Number: | 9023-88-5 |
Synonyms: | mannose-6-phosphate isomerase; D-mannose-6-phosphate aldose-ketose-isomerase |
Source: | Escherichia coli |
Molecular Weight: | 43,915 |
Concentration: | Supplied at ~ 500 U/mL |
Expression: | Recombinant from Escherichia coli |
Specificity: | Catalyses the reaction: D-Mannose 6-phosphate = D-fructose 6-phosphate |
Specific Activity: | ~ 100 U/mg (25oC, pH 7.6 on mannose 6-phosphate) |
Unit Definition: | One Unit of phosphomannose isomerase activity is defined as the amount of enzyme required to release one µmole of fructose 6-phosphate from mannose 6-phosphate (3.14 mM) per minute in the presence of NAD+ at pH 7.6 and 25oC. |
Temperature Optima: | 40oC |
pH Optima: | 7.6 |
Application examples: | Applications for the measurement of D-mannose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |