Phosphotransacetylase (Bacillus subtilis)

Phosphotransacetylase (Bacillus subtilis)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-PTABS

SKU: EA-261-143

Description

Phosphotransacetylase (Bacillus subtilis)

Phosphotransacetylase (Bacillus subtilis)

Content: 3,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Other Activities
EC Number: 2.3.1.8
CAS Number: 9029-91-8
Synonyms: phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase
Source: Bacillus subtilis
Molecular Weight: 36,953
Concentration: Supplied at ~ 3,000 U/mL
Expression: Recombinant from Bacillus Subtilis
Specificity: Catalyses the reaction:
Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate
Specific Activity: ~ 3,500 U/mg (25oC, pH 7.4 on Coenzyme A)
Unit Definition: One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25oC.
Temperature Optima: 25oC
pH Optima: 7.4
Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.
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