Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

MANUFACTURER: MEGAZYME

PRODUCT CODE: K-PullG6

SKU: EA-261-105

Description

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

Content: 100 / 200 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Pullulanase/Limit-Dextrinase
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: 0.18 U/mL for pullulanase preparations (50-fold dilution)
0.01 U/g for limit dextrinase in milled malt
Reproducibility (%): ~ 3%
Total Assay Time: ~ 10 min (Pullanase),
~ 30 min (Limit-Dextrinase)
Application examples: Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
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