Succinyl-CoA synthetase (prokaryote)

$550.22

Succinyl-CoA synthetase (prokaryote)

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-SCOAS

SKU: EA-261-146

Description

Succinyl -CoA synthetase (prokaryote)

Succinyl-CoA synthetase (prokaryote)

Content: 550 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions

High purity recombinant Succinyl -CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Enzyme Activity: Other Activities
EC Number: 6.2.1.5
CAS Number: 9080-33-5
Synonyms: succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)
Source: Prokaryote
Molecular Weight: 30,843,
41,393
Concentration: Supplied at ~ 220 U/mL
Expression: Recombinant from a Prokaryotic source
Specificity: Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Specific Activity: ~ 9 U/mg (25oC, pH 8.4 on succinic acid)
Unit Definition: One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one µmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25oC.
Temperature Optima: 25oC
pH Optima: 8.4
Application examples: Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.