Harlequin EColi/Coliform Agar
Harlequin EColi/Coliform Agar

Tryptic Soy Agar (Soybean-Casein Digest Agar)

Harlequin EColi/Coliform Agar Half Fraser (Demi-Fraser) Broth (ISO)
Harlequin EColi/Coliform Agar Buffered Peptone Water (BPW)

$140.35

Manufacturer Name: NEOGEN

Manufacturer No: NCM0002A

 

 

 

 

 

 

 

SKU: EA-1613-378
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Description

Tryptic Soy Agar (Soybean-Casein Digest Agar)

 

Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. It is also a support plating medium for various protocols described in the microbial enumeration test section of the Harmonized USP/EP/JP. Tryptic Soy Agar is not intended for use in the diagnosis of disease or other conditions in humans. Enzymatic digests of casein and soybean act as a source of nitrogen and amino acid and sodium chloride maintains the osmotic balance.

 

Specifications
Brand Neogen® Culture Media
Analyte Non-selective
Platform Dehydrated Culture Media
Mass 5 kg
Formula
Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soybean 5.0 g/L
Sodium Chloride 5.0 g/L
Agar 15.0 g/L
Grams per Liter 40.00
Liters per 500 g 13.00
Plates per 500 g 680.00
Preparation
  1. Suspend 40 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7100 Tryptic Soy Agar; and LabM Product: HP016 Casein Soya Bean Digest Agar (USP/EP/JP)
Package Weight 14.00 lb

Specifications
Brand Neogen® Culture Media
Analyte Listeria spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Peptone Mixture 15.0 g/L
Esculin 1.0 g/L
Disodium Hydrogen Phosphate 9.6 g/L*
Potassium Dihydrogen Phosphate 1.35 g/L
Sodium Chloride 20.0 g/L
Lithium Chloride 3.0 g/L
Acriflavine 0.0125 g/L
Nalidixic Acid 0.01 g/L

*Equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/L
Grams per Litre 55.00
Litres per 500 g 9.00
Preparation
  1. Dissolve 55 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes 4. Cool to 45-50°C.
  4. Aseptically add 2 vials of NCM4009-0.5* Ferric Ammonium Citrate Supplement.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.
Previous Item Number Product was previously known as Acumedia Product: 7656 Demi-Fraser Broth Base; and LabM Product: LAB211 Half Fraser Broth PLUS
Package Weight 1.35 lb

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