Xylanase Assay Kit (XylX6 Method)

Description

Xylanase Assay Kit (XylX6 Method)

Xylanase Assay Kit (XylX6 Method)

The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Content: (K-XylX6-1V)
100 assays (manual) / 200 assays (auto-analyser)
(K-XylX6-2V)
200 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: endo-1,4-β-Xylanase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400,
405
Signal Response: Increase
Limit of Detection: 5.3 x 10-4 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: 10 min
Application examples: Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.
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