Description

Zearalenone Test  Card    

Principle

Zearalenone test card is based on lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Zearalenone in the sample, a visible T band will appear; T band is invisible if it has more Zearalenone in the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Zearalenone in the Edible oil ,feed,corn,wheat,grains.

 

Technical Specification                                                

Reaction temperature: 25℃

Detect limit： 10µg/kg

 

Materials provided                                                

Rapid Test Card	20PCS
Sample Extraction	50mL

 

 

Materials required not provided

Micropipettors:  100~ 1000 µL

Equipment:Vortex, Balance (0.01 g)，Centrifuge,  Balance(0.01g), Hot bath , Shaker

 

Sample Preparation   

–Grains~rice,corn,wheat,peanut                                          

• Take 1±05g of the homogenized sample, put into 50mL centrifuge tube, add

2mL sample extraction,vortex for 2min.

• Centrifuge at above 4000r/min at room temperature (20-25℃) for 5 min.

[Or extract at room temperature for 15 min without Centrifuge].

• Take 80µL upper layer for analysis.

 

–Edible oil                                          

• Take 1±05g edible oil , put into 10mL centrifuge tube, add 1mL sample

extraction,vortex for 2min.

• Centrifuge at above 4000r/min at room temperature (20-25℃) for 5 min.

[Or extract at room temperature for 15 min without Centrifuge].

• Take 80µL lower layer for analysis.

 

–Liquid milk

1. Take 100µL liquid milk ,add 100µL sample extraction,vortex for 30s.
2. Take 80µL for analysis.

 

Detect process

1. Take out the cassette from the pack and place it horizontally.
2. Return to room temperature (20-25℃), determine the cassette immediately.
3. Add 2 drops sample solution（About 80µL, react for 10 min and read result.

 

Result Judgement

 

1. Positive:

The presence of “C” band, no matter T band. It means that aflatoxin(AFB1)

in the sample extraction is higher than 500µg / kg .

 

2. Negative:

Both clear“C” band and “T” band appears. It means that aflatoxin(AFB1)

in the sample extractionis lower than 500µg / kg .

 

3. Invalid:

No colored band appears in C zone, no matter whether T band appears.

Note                                                            

1. For best results, please strictly adhere to these instructions.
2. All reagents must be at room temperature before running the assay.
3. Do not remove test cassette from its pouch until immediately before use.
4. Do not reuse the test kit.
5. Do not use the test beyond its expiration date marked on the foil pouch.
6. The components in this kit have been quality control tested as standard batch unit.
7. Do not mix components from different lot numbers.

 

Storage and expiry date                                             

1. Store at 4-30 °C for 12 months in the dark. Do not freeze.
2. Expiry date is labelled on the package.