Acetylxylan esterase (Orpinomyces sp.)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-AXEAO-1KU
- Description
- Size Guide
- Specification
- DATA SHEET
Description
Acetylxylan esterase (Orpinomyces sp.)
Content: | 1,000 Units or 3,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
High purity recombinant Acetylxylan esterase (Orpinomyces sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Enzyme Activity: | Esterase |
EC Number: | 3.1.1.72 |
CAZy Family: | CE6 |
CAS Number: | 188959-24-2, 9000-82-2 |
Synonyms: | acetylxylan esterase |
Source: | Orpinomyces sp. |
Molecular Weight: | 34,000 |
Concentration: | E-AXEAO-1KU: Supplied at ~ 1,000 U/mL. E-AXEAO-3KU: Supplied at ~ 1,000 U/mL. |
Expression: | Recombinant from Orpinomyces sp. |
Specificity: | Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. |
Specific Activity: | ~ 36 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate) |
Unit Definition: | One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40oC. |
Temperature Optima: | 40oC |
pH Optima: | 7 |
Application examples: | Applications established in carbohydrate research and biofuel industries. |