Acetylxylan esterase (Orpinomyces sp.)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-AXEAO-1KU
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Description
Acetylxylan esterase (Orpinomyces sp.)
| Content: | 1,000 Units or 3,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
High purity recombinant Acetylxylan esterase (Orpinomyces sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Enzyme Activity: | Esterase |
| EC Number: | 3.1.1.72 |
| CAZy Family: | CE6 |
| CAS Number: | 188959-24-2, 9000-82-2 |
| Synonyms: | acetylxylan esterase |
| Source: | Orpinomyces sp. |
| Molecular Weight: | 34,000 |
| Concentration: | E-AXEAO-1KU: Supplied at ~ 1,000 U/mL. E-AXEAO-3KU: Supplied at ~ 1,000 U/mL. |
| Expression: | Recombinant from Orpinomyces sp. |
| Specificity: | Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. |
| Specific Activity: | ~ 36 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate) |
| Unit Definition: | One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7 |
| Application examples: | Applications established in carbohydrate research and biofuel industries. |
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