Nutrient medium Lactose Broth
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Nutrient Medium
Type:Lactose Broth
Manufacturer: Dr. Möller & Schmelz GmbH (M&S)
Item Number:5040
Packaging: 4x100ml (2x concentration)
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Description
NUTRIENT MEDUIM-Lactose Broth
DESCRIPTION AND APPLICATION RANGE
Lactose-Broth is used for the enrichment and the detection of E. coli and coliforms from water and other samples(see Mineral-und Tafel Wasser verordnung (mineral-and table water regulation).E. Coli and coliforms are able to ferment the carbon source Lactose. During this process acidic metabolites and CO2 are formed. Due to the acids the pH –value of the medium drops down causing a color change of the pH –indicator bromocresolpurple from purple to yellow. The formation of CO is detected with the Durham –tube, in which the gas is collected.The medium is manufactured and quality tested in compliance with DIN EN ISO 11133:2014 standard
Profile: Bottles
Storage: Dark and dry at 4-12°C
Colour: Beige
Shelf life: 6 months
INTRODUCTION
M&S sterile ready-to-use media are filled in autoclavable and break-proof polycarbonate bottles (50 ml and 250 ml) or glass tubes (20 ml). The composition of the various types of media, comply, like the NPS with the formulations specified in the pertinent standards and regulations.
Whereas the liquid media can be used immediately, the agar media first have to be remelted in a water bath at 90 – 100 °C. Once they have cooled down to around 50 °C you can pour agar plates in different sizes and thicknesses to meet your individual demands.
The shelf life of the ready-to-use media in unopened containers is 6 months.
Generally the M&S ready-to-use media are offered in three different pack sizes.
Standard pack sizes:
- 4 x 250 ml polycarbonate bottles
- 25 x 50 ml polycarbonate bottles
- 25 x 20 ml glass tubes
QUALITY ASSURANCE
Just like the nutrient pad sets, the production processes and quality control for our ready-to-use nutrient media
are integrated in the M&S quality management system and are based on the GMP guidelines.
The broth and agar media are produced using components from certified suppliers only. The weighed portions
of the individual components are documented with their batch numbers to guarantee their traceability
and the adjustment of the pH-value is also recorded. Careful filling and steriliZation ensures that the quality
of the individual nutrient media batches remain unaffected.
During the final control of the end products, sterility, recovery rates and if necessary any colour reactions
with positive and negative microorganisms are tested. The nutrient media only leave our premises if they fulfil
all of the requirements.
The batch-related M&S quality certificate that is enclosed with every package confirms that M&S products
have been checked according to our quality standards and that they meet these.
