[Cross Reactivity]
Ofloxacin (OFL) ······················································100%
Ciprofloxacin (CIP) ··················································· <1%
Enrofloxacin (ENR) ·····················································2%
Norfloxacin (NOR) ····················································<1%
Salafloxacin (SAR) ····················································<1%
Pefloxacin (PEF) ························································ 3%
Lomefloxacin (LOM) ················································· <1%
Danfloxacin (DAN) ··················································· <1%
Enoxacin (ENO) ······················································· <1%
Mapofloxacin (MAR) ················································130%
Difloxacin (DIF) ······················································· 28%
Flumequine ·····························································<1%
Nafloxacin ······························································<1%
Oxalic Acid ·····························································<1%
Obifloxacin ·····························································<1%
[Materials Required But Not Provided]
- Devices:
—- Microplate Reader (450nm/630nm filter)
—- Vortex
—- Constant Temperature Incubator (Optional)
—- Centrifuge
—- Homogenizer
—- Balance (sensibility: 0.01g)
—- Rubber Bulb Syringe
—- Termovap Sample Concentrator
—- Centrifuge tube: 5mL/7mL, 50mL
—- Graduated Pipette: 10mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
- Reagent:
—- Deionized Water
—- Acetonitrile (AR)
—- N-hexane (AR)
—- Concentrated Hydrochloric Acid (HCl) (AR)
—- 18M Concentrated Sulfuric Acid (H2SO4)
[Provided Materials and Reagent]
| Component | 96 wells |
| Antigen Coated Plate | 96T |
| 5 vials of Standard | 1mL each |
| High Concentration Standard (100ppb) | 1mL |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated Sample Diluting Buffer | 15mL |
| Package Insert | 1 |
| Testing Report | 1 |
| Plate Cover Sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb.












