BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L)
Monoclonal Mouse Anti-Human Ig Kappa
BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L)
Monoclonal Mouse Anti-Human Ig Kappa

Paraquat Rapid Test Kit

BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L) Paclobutrazol Rapid Test Kit
BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L) Parathion-methyl Rapid Test Kit

$333.50

Manufacturer: Ecalbio

 

 

SKU: EA-137-382
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Description

Paraquat Rapid Test Kit    

Paraquat Rapid Test Kit

Principle of Paraquat Rapid Test Kit

Paraquat rapid test kit is based on lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Paraquat in the sample, a visible T band will appear; T band is invisible if it has more Paraquat in the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Paraquat in the fruit, vegetables, grains,milk sample.

 

Technical Specification  for Paraquat Rapid Test Kit

                                        

Detect limit：  2ppb            Reaction temperature: 25℃

Detect time:   30min.

 

Materials provided  for Paraquat Rapid Test Kit

                                              

Rapid Test Card 20PCS
PBS solution     50mL

 

Materials required not provided  for Paraquat Rapid Test Kit

                                     

Deionized water, Ethyl acetate

Centrifuge tube:  5mL, 10mL

Micropipettors:  20~200 µL, 100~ 1000 µL

Vortex, centrifuge, balance (0.01 g), homogenizer, shaker

 

Sample Preparation  of Paraquat Rapid Test Kit

                                         

  1. Take 5±05g of the homogenized sample, add 5mL deionized water, then add

5mL ethyl acetate , shake for 5min properly.

  1. Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 10 min.
  2. Transfer 3mL supernatant, evaporate to dryness by nitrogen at 50~60 ℃.
  3. Dissolve the dry residue in 200uL PBS solution , mix for 30s.
  4. Take 80µL for analysis.

 

Sample Determination  of Paraquat Rapid Test Kit

                                                                                   

  1. Take out the cassette from the pack and place it horizontally.
  2. Return to room temperature (20-25℃), determine the cassette immediately.
  3. Add 2 drops(Approx 80µL) sample extraction, react for 5-10 min and read result.

Result Judgement for Paraquat Rapid Test Kit

  1. Positive: The presence of “C” band, no matter “T”

It means that Paraquat in the sample extraction is higher than 2ppb.

  1. Negative: Both clear“C” band and “T” band appears.

It means that Paraquat in the sample extractionis lower than 2ppb .

  1. Invalid: No colored band appears in C zone, no matter whether T band

appears.

Note                                                             

  1. For best results, please strictly adhere to these instructions.
  2. All reagents must be at room temperature before running the assay.
  3. Do not remove test cassette from its pouch until immediately before use.
  4. Do not reuse the test kit.
  5. Do not use the test beyond its expiration date marked on the foil pouch.
  6. The components in this kit have been quality control tested as standard batch unit.
  7. Do not mix components from different lot numbers.

 

Storage and expiry date for Paraquat Rapid Test Kit

                                        

  1. Store at 4-30 °C for 12 months in the dark. Do not freeze.
  2. Expiry date is labelled on the package.

 

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