Parathion-methyl Rapid Test Kit
$333.50
Manufacturer: Ecalbio
- Description
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Description
Parathion-methyl Rapid Test Kit
Principle of Parathion-methyl Rapid Test Kit
Parathion-methyl rapid test kit is based on lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Parathion-methyl in the sample, a visible T band will appear; T band is invisible if it has more Parathion-methyl in the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Parathion-methyl in the fruit, vegetables, grains,milk sample.
Technical Specification of Parathion-methyl Rapid Test Kit
Detect limit: 500ppb Reaction temperature: 25℃
Detect time: 30min.
Materials provided for Parathion-methyl Rapid Test Kit
Rapid Test Card | 20PCS |
PBS solution | 50mL |
Materials required not provided for Parathion-methyl Rapid Test Kit
Deionized water, Ethyl acetate
Centrifuge tube: 5mL, 10mL
Micropipettors: 20~200 µL, 100~ 1000 µL
Vortex, centrifuge, balance (0.01 g), homogenizer, shaker
Sample Preparation of Parathion-methyl Rapid Test Kit
- Take 5±05g of the homogenized sample, add 5mL deionized water, then add
5mL ethyl acetate , shake for 5min properly.
- Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 10 min.
- Transfer 3mL supernatant, evaporate to dryness by nitrogen at 50~60 ℃.
- Dissolve the dry residue in 200uL PBS solution , mix for 30s.
- Take 80µL for analysis.
Sample Determination of Parathion-methyl Rapid Test Kit
- Take out the cassette from the pack and place it horizontally.
- Return to room temperature (20-25℃), determine the cassette immediately.
- Add 2 drops(Approx 80µL) sample extraction, react for 5-10 min and read result.
Result Judgement of Parathion-methyl Rapid Test Kit
- Positive: The presence of “C” band, no matter “T”
It means that Parathion-methyl in the sample extraction is higher than 500ppb.
- Negative: Both clear “C” band and “T” band appears.
It means that Parathion-methyl in the sample extractionis lower than 500ppb .
- Invalid: No colored band appears in C zone, no matter whether T band
appears.
Note
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not reuse the test kit.
- Do not use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit.
- Do not mix components from different lot numbers.
Storage and expiry date of Parathion-methyl Rapid Test Kit
- Store at 4-30 °C for 12 months in the dark. Do not freeze.
- Expiry date is labelled on the package.