Phosphatase (Acid) (Escherichia coli)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-ACPEC
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Description
Phosphatase (Acid) (Escherichia coli)
Product Highlight for Phosphatase (Acid) (Escherichia coli)
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC |
| Enzyme Activity: | Phosphatase |
| EC Number: | 3.1.3.2 |
| CAS Number: | 9001-77-8 |
| Synonyms: | acid phosphatase; phosphate-monoester phosphohydrolase (acid optimum); orthophosphoric-monoester phosphohydrolase (acid optimum) |
| Source: | Escherichia coli |
| Molecular Weight: | 24,667 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
| Specific Activity: | ~ 14 U/mg (40oC, pH 5.0 on 4-Nitrophenyl phosphate) |
| Unit Definition: | One Unit of acid phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl phosphate (4 mM) in sodium acetate buffer (20 mM), pH 5.0 at 40oC. |
| Temperature Optima: | 45oC |
| pH Optima: | 5 |
| Application examples: | Applications in research including molecular biology and biocatalysis. |
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