Phosphatase (Alkaline) (Escherichia coli)
MANUFACTURER: MEGAZYME
PRODUCT CODE; E-ALPEC
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Description
Phosphatase (Alkaline) (Escherichia coli)
Product Highlight for Phosphatase (Alkaline) (Escherichia coli)
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC |
| Enzyme Activity: | Phosphatase |
| EC Number: | 3.1.3.1 |
| CAS Number: | 9001-78-9 |
| Synonyms: | alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum) |
| Source: | Escherichia coli |
| Molecular Weight: | 50,503 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
| Specific Activity: | ~ 10 U/mg (37oC, pH 10.4 on p-nitrophenyl phosphate) |
| Unit Definition: | One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 10.4 |
| Application examples: | Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA). |
