Phosphotransacetylase (Bacillus subtilis)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-PTABS
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Description
Phosphotransacetylase (Bacillus subtilis)
Product Highlight for Phosphotransacetylase (Bacillus subtilis)
| Content: | 3,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. |
| Enzyme Activity: | Other Activities |
| EC Number: | 2.3.1.8 |
| CAS Number: | 9029-91-8 |
| Synonyms: | phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase |
| Source: | Bacillus subtilis |
| Molecular Weight: | 36,953 |
| Concentration: | Supplied at ~ 3,000 U/mL |
| Expression: | Recombinant from Bacillus Subtilis |
| Specificity: | Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate |
| Specific Activity: | ~ 3,500 U/mg (25oC, pH 7.4 on Coenzyme A) |
| Unit Definition: | One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.4 |
| Application examples: | Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries. |
