Protease (Subtilisin A from Bacillus licheniformis)
$578.54
Protease (Subtilisin A from Bacillus licheniformis)
MANUFACTURER: MEGAZYME
PRODUCT CODE: E-BSPRT-40ML
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Description
Protease (Subtilisin A from Bacillus licheniformis)
| Content: | 0.5 grams – 10 mL or 2 grams – 40 mL or 5 grams – 100 mL or 2.5 grams – 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Dietary Fiber test method.
E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Enzyme Activity: | Protease |
| EC Number: | 3.4.21.62 |
| CAS Number: | 9014-01-1 |
| Synonyms: | subtilisin; subtilisin A |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 30,250 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
| Specific Activity: | ~ 6 U/mg of protein (40oC, pH 8.0 on casein) |
| Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
