BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L)
Monoclonal Mouse Anti-Human Ig Kappa
BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L)
Monoclonal Mouse Anti-Human Ig Kappa

Tetrodotoxin (TTX) Test Kit

BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L) Trimethoprim Rapid Test Kit
BIOTIN*Polyclonal Goat Anti-Mouse IgG (H+L) Tylosin Rapid Test Kit

$333.50

Manufacturer: Ecalbio

 

 

SKU: EA-137-408
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Description

Tetrodotoxin (TTX) Test Kit   

 HRP-SA

Principle

Tetrodotoxin(TTX) rapid test kit is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is little Tetrodotoxin (TTX) in the sample, a visible T band will appear; T band is invisible if it has more Tetrodotoxin (TTX) in the sample. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Tetrodotoxin (TTX) in the sample.

 

 

Technical Specification                                                

Reaction temperature: 25℃

Detect limit： 0.22µg/mg

Detect time:  30min.

 

Materials provided                                                

Rapid Test Card 30PCS
PBS Tablets     5

 

Materials required not provided

Deionized water

Centrifuge tube:  50mL, 10mL

Micropipettors:  20~200 µL, 100~ 1000 µL

Centrifuge, Balance(0.01g), Hot bath , Shaker

 

Sample diluent (PBS solution , pH=7.2)

1 PBS Tablet , dissolve in 100mL deionized water shake properly.

 

Sample Preparation                                             

Take 1 ± 0.05 g of the homogenized sample, add 3 mL sample diluent , extracted in the hot bath for 10 min, then cool to room temperature (20-25℃), determine the sample immediately.

 

Detect process

  1. Take out the cassette from the pack and place it horizontally.
  2. Return to room temperature (20-25℃), determine the cassette immediately.
  3. Add 3drops (Approx 90µL) sample extraction, react for 10 min and read result, if

there is no color presence after adding 1 drops sample extraction, add to one drop to original device.

Result Judgement

 

  1. Positive:

The presence of “C” band, no matter T band. It means that Tetrodotoxin (TTX)

in the sample extraction is higher than 1 MU/mL (0.22 µg) .

  1. Negative:

Both clear“C” band and “T” band appears. It means that Tetrodotoxin (TTX)

in the sample extractionis lower than 0.25 MU/mL (0.05 µg) .

  1. Invalid:

No colored band appears in C zone, no matter whether T band appears.

  1. Precautions:

Determine the result within 10 min, if the result is higher than 10 MU/g (Fold of dilution of the sample is 10, the resulting value is subsequently multiplied by the dilution fold), it means the sample is positive, the sample is negative if the result less than 10 MU/g.

 

Note                                                            

  1. For best results, please strictly adhere to these instructions.
  2. All reagents must be at room temperature before running the assay.
  3. Do not remove test cassette from its pouch until immediately before use.
  4. Do not reuse the test kit.
  5. Do not use the test beyond its expiration date marked on the foil pouch.
  6. The components in this kit have been quality control tested as standard batch unit.
  7. Do not mix components from different lot numbers.

 

 

Storage and expiry date                                             

  1. Store at 4-30 °C for 12 months in the dark. Do not freeze.
  2. Expiry date is labelled on the package.

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