Amyloglucosidase (Aspergillus niger) Powder

Amyloglucosidase (Aspergillus niger) Powder

MANUFACTURER: MEGAZYME

PRODUCT CODE: E-AMGDFPD

SKU: EA-261-155

Description

Amyloglucosidase (Aspergillus niger) Powder

Amyloglucosidase (Aspergillus niger) Powder

Content: 5.5 g – 26,000 Units/g
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: > 1 year under recommended storage conditions

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase  and protease powder. For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.

Also suitable for use in AOAC 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004.

Enzyme Activity: Amyloglucosidase
EC Number: 3.2.1.3
CAZy Family: GH15
Synonyms: 4-alpha-D-glucan glucohydrolase; glucoamylase
Source: Aspergillus niger
Molecular Weight: 143,500
Expression: From Aspergillus niger
Specificity: Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.
Specific Activity: ~ 26,000 U/g (40oC, pH 4.5, soluble starch as substrate).
~ 2,260 U/g on p-nitrophenyl β-maltoside
Unit Definition: One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC.
Temperature Optima: 70oC
pH Optima: 4
Method recognition: AOAC Method 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004
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